资源下载

科学仪器目录

应用文献-The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs

已下载1380次 / 原平皓生物
*如果您在实验中遇到任何问题, 请与我们的技术人员沟通, 我们将提供帮助.
联系电话: 010-5158 2336转803 电子邮箱: helpyph-bio.com

简介:

The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs
Katsutomo Okamura1, Wei-Jen Chung1, J. Graham Ruby2, Huili Guo2, David P. Bartel2 & Eric C. Lai1

In contrast to microRNAs and Piwi-associated RNAs, short interfering RNAs (siRNAs) are seemingly dispensable for host-directed gene regulation in Drosophila. This notion is based on the fact that mutants lacking the core siRNA-generating enzyme Dicer-2 or the predominant siRNA effector Argonaute 2 are viable, fertile and of relatively normal morphology1,2. Moreover, endogenous Drosophila siRNAs have not yet been identified. Here we report that siRNAs derived from long hairpin RNA genes (hpRNAs) programme Slicer complexes that can repress endogenous target transcripts.
The Drosophila hpRNA pathway is a hybrid mechanism that combines canonical RNA interference factors (Dicer-2, Hen1 (known as CG12367) and Argonaute 2) with a canonical microRNA factor (Loquacious) to generate 21-nucleotide siRNAs. These novel regulatory RNAs reveal unexpected complexity in the sorting of small RNAs, and open a window onto the biological usage of endogenous RNA interference in Drosophila.